Chad Markert – Mouse Limb Scan

10/22/09


Google Presentation

 

Organic Filter Video


video2 SVRT from Web Master on Vimeo.

Here an organic filter is used to give the mouse joint a more realistic appearance.

 

Mouse Knee Video 2

video3 VRT from Web Master on Vimeo.Another view of the mouse knee demonstrates how the vasculature curves around the bones in the knee joint.

Mouse Knee Axial Video

topdown from Web Master on Vimeo.

This axial video shows the arrangement of the vasculature and bone structures in the knee.

Scanner Settings
Machine Xradia microXCT
Binning
1
Source Setting
40 kv 200 µA
Source Distance
60 mm
Detector Distance 35 mm
Pixel Size
2.1654 µm
FOV 4.43 mm
Magnification 3.938 X
Exposure Time 60 s
Image Size 2048 X 2048
Number of Projections 271

 

Summary

Dr. Chad Markert of Wake Forest University was interested in the effects of the introduction of certain drugs on mouse vasculature. To study these effects, he sent samples of mouse limbs to Virginia Tech’s Multiscale CT Facility to be scanned by Kriti Sen Sharma. By using the appropriate settings, the vasculature of the mouse limb was able to be shown in great detail.

Subject

Although the object being scanned is a mouse limb, the main focus of the scan was the veins in the limb and the effects that drug injections would have.

About the Scan

The sample arrived to Virginia Tech’s Multiscale CT Facility soaked in a formalin solution. This solution preserved the mouse limb and kept the vein structure intact. Because it was only necessary to view the vein structure, deterioration of the musculature did not affect the results. In keeping with the focus on the mouse’s veins near the knee joint, only the knee was scanned.