Joseph Freeman – Bone Tissue Scaffolding Scan

10/01/09


Google Presentation

Video:

3D Hue Shading Video

green movie from Web Master on Vimeo. This 3D rendering of the scaffold utilizes a hue shader which makes the scaffolding more visible compared to the black and white shader.

Axial Scaffold Video

topdown from Web Master on Vimeo. This axial section video shows the concentricity of the fibers.

Scanner Settings
Machine Xradia microXCT
Binning
1
Source Setting
30 kv 100 µA
Source Distance
55 mm
Detector Distance 20 mm
Pixel Size
2.5140 µm
FOV 5.15 mm
Magnification 3.938 X
Exposure Time 60 s
Image Size 2048 X 2048
Number of Projections 271

Summary

CT imaging is very useful in the development of bone scaffolding because it is necessary to see the scaffold in its entirety.  Other methods such as a Scanning Electron Microscope (SEM) only offer surface imaging and require destructive sectioning of the sample. In this case, CT imaging allows us to see osteon like scaffolds on which cortical bone tissue can grow. Knowledge of artificial bone tissue scaffolding will be useful in developing bone grafting procedures for serious injuries.

Subject

The subject of the scan is a 3D scaffold made of Poly-L-lactide fibers on polyglycolide fibers. The fibers are spin together by electrospinning, then they are molded and sintered into the desired shape.

About the Scan

271 images were taken at 60 seconds per projection. The scan used a sweep angle of +- 135 degrees. Source voltage was at 30 kV with a current of 100 microamps. Each scan took approximately 5 hours. Samples were scanned in a .5 mL PCR tube. Scanning was performed by Kriti Sen Sharma.

Figure: Samples in their PCR tubes.

Links and Literature

Powerpoint presentation about the use of Micro CT in researching bone scaffolds:http://sites.google.com/site/kritisen/files/ncterm_kriti_ver2.pptx?attredirects=0